Urine collection

1. Collection of morning midstream urine specimen

  • The first morning midstream urine is suitable for analysis.
  • The time between the collection of urine and last urination must be at least 4-6 hours.
  • Before giving the sample eating or drinking is not allowed for at least 8 hours. If necessary, one glass of pure water may be drunk.
  • Only to collect the urine in a single plastic sample container for this purpose. Sampling equipment can be obtained from a doctor or lab.
  • We ask you not to bring the analyses to the lab in everyday containers.
  • Urine should be collected directly into the sampling container. Pouring urine from one container to another is not permitted.
  • During the collection of the sample beware of touching the inside of the container or its lid.
  • All sampling equipment (cups, tubes, straw) are disposable.
  • Before sampling the urine wash the genital organs with warm, running water; detergents (soap, etc.) are not allowed. It is important to clean the outer urethra – in order to do this, in females spread the labia with fingers; in males pull back the foreskin.
  • Dry the external genitals mildly with a paper towel.
  • If women have menstrual blood excretions, use a tampon.
  • During urination make sure that the urethra is uncovered during the sampling.
  • Urinate a couple of millilitres into the toilet, then urinate continuously (about 50 ml) into the sample container and finish urinating in the toilet pot.
  • After sampling the urine, close the lid of the sample container properly.
  • Special plastic bags may be used in infants and toddlers for collecting the samples. The external genital area of the child must be cleaned, then attach the bag and monitor 10-15 minutes for urine formation. Urine should be transferred from the bag with a special straw into the sampling container.
  • The container should be labelled.
  • Keep the collected urine at +2 ° to +8 ° C.

2. First urine collection for molecular diagnostics

  • Urine can only be used in molecular diagnostics in men.
  • Prior to taking the sample the external genitals may not be washed.
  • Take the first 3–5 ml of urine for analysis from morning urination.
  • The container should be labelled.

3. 24-hour urine collection

  • The 24-hour urine is collected into a clean container that can be closed with a lid and has a scale (capacity of 2-3 litres). The container can be obtained from a doctor or lab.
  • In the morning of the first day of urine collection empty the bladder into the toilet pot, fix the time of urination on the referral letter (beginning of urine collection).

NB! In analyses for catecholamine, calcium, magnesium and phosphate, preservative of concentrated hydrochloric acid (HCl – has a strong corrosive effect) is added after the first urine sample into the container.

  • Hydrochloric acid is given from the lab along with the container.
  • All of the following urine samples should be collected into the container; no amount of urine may be lost.
  • Keep the container between urination in a cool and dark place.
  • After adding each following urine sample, the urine should be mixed.
  • The last urine sample should be taken on the next day in the morning at the same time as the beginning of the collection; the collection end time should be fixed on the referral letter.
  • Measure the quantity of 24 h urine by the scale on the container and note it to the referral letter.
  • You may bring the whole container with urine or about 50 mL in a sampling container that is provided by the lab (mix the collected urine carefully before sampling).
  • The container should be labelled.

For questions, please contact your doctor or nurse or laboratory, by phone at 605 0758.